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Analysis of multiplex PCR fragments with PMMA microchip |
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| Authors: | Liu Dayu Zhou Xiaomian Zhong Runtao Ye Nannan Chang Guohui Xiong Wei Mei Xiaodan Lin Bingcheng |
| Affiliation: | a Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Zhongshan Road 457, Postcode 116023, Dalian, China b Department of Clinical Laboratory, Guangzhou First Municipal People's Hospital, Affiliated of Guangzhou Medical College, 510500 Guangzhou, PR China c Graduate School of the Chinese Academy of Sciences, Beijing 100000, PR China d Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Postcode 100071, Beijing, China |
| Abstract: | Microchip electrophoresis is a promising technique for analysis of bio-molecules. It has the advantages of fast analysis, high sensitivity, high resolution and low-cost of samples. Plastic chip has the potential of mass production for clinical use for its advantages in biocompatibility and low cost. In this work, the method for fabrication of poly(methyl methacrylate) (PMMA) chip was described, and conditions for DNA separation were investigated with the chip. The PMMA microchip was used for detection of multiplex PCR products of 18 and 36 cases with SARS and hepatitis B virus infection under optimized separation conditions. Microchip electrophoresis showed higher sensitivity, higher resolution and less time consumption when compared with gel electrophoresis. The microchip electrophoresis with PMMA chip provided a rapid, sensitive and reliable method for analysis of multiplex PCR products. |
| Keywords: | HPMC, hydroxyprolymethyl cellulose PDMS, poly(dimethylsiloxane) PMMA, poly(methyl methacrylate) SARS, severe acute respiratory syndrome |
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