Enhanced optical immunosensor based on surface plasmon resonance for determination of transferrin |
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Authors: | Liu Xia Sun Ying Song Daqian Zhang Qinglin Tian Yuan Zhang Hanqi |
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Affiliation: | College of Chemistry, Jilin University, Changchun 130012, PR China |
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Abstract: | Wavelength modulation surface plasmon resonance biosensors (SPR) using colloidal Au nanoparticles and double-linker sensing membrane enhancement are reported for determination of transferrin. The 2-mercaptoethylamine (MEA) was immobilized on the biosensor surface with traditional amine coupling method. The interaction between colloidal Au nanoparticles and MEA was investigated. The anti-transferrin was immobilized on the biosensor surface prepared with staphylococcal protein A (SPA). The interaction of the antibody and antigen was monitored in real time. The good response was obtained in the concentration range 1-20, 0.1-20 and 0.05-20 μg/mL for directly immune assay, double-linker assay and colloidal Au-amplified assay. The result clearly demonstrates that these methods may obtain significantly enhancement of sensitivity for the wavelength modulation SPR biosensor. |
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Keywords: | SPR, surface plasmon resonance SPA, staphylococcal protein A PBS, phosphate-buffered saline BSA, bovine serum albumin MPA, 3-mercaptopropionic acid EDC, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide NHS, N-hydroxysuccinimide MEA, 2-mercaptoethylamine |
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