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Relationship between adsorbed fibronectin and cell adhesion on a honeycomb-patterned film |
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| Authors: | Sadaaki Yamamoto Masaru Tanaka Hiroshi Sunami Keiko Arai Shigeko Yamashita Masatsugu Shimomura |
| Institution: | a Creative Research Initiative “Sousei” (CRIS), Hokkaido University, N21W10 Kita-ku, Sapporo 001-0021, Japan b Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Honchou 4-1-8, Kawaguchi 332-0012, Japan c Spatio-Temporal Function Materials Research Group, Frontier Research System, RIKEN Institute, 1-12 Hirosawa, Wako, Saitama 351-0198, Japan d Nanotechnology Research Center, Research Institute for Electronic Science, Hokkaido University, N12W6 Kita-ku, Sapporo 060-0812, Japan |
| Abstract: | Substratum surface morphology plays a vital roles in cellular behavior. Here, we characterized adsorption of fibronectin (Fn) as a typical cell adhesion protein onto honeycomb-patterned films made of poly(ε-caprolactone) (PCL) by using atomic force microscopy (AFM) and confocal laser scanning microscopy (CLSM). In order to determine how cells adhere to a honeycomb-patterned film, focal adhesion of cardiac myocytes (CMYs) and endothelial cells (ECs) on the films were studied by using fluorescence labeling of vinculin. Fn adsorbs around the pore edges to form ring-shaped structures. CMYs and ECs adhere onto the honeycomb-patterned films at focal contact points localized around pore edges distributed over the entire cellular surface. The focal contact points on the honeycomb-patterned films correspond well with the adsorption sites of Fn. We suggest that the cell response to honeycomb-patterned films is associated with the adsorption pattern of Fn on the film. |
| Keywords: | Atomic force microscopy Scanning electron microscopy Cell adhesion Adsorption Self-assembly Surface topography Fibronectin Tissue engineering |
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