CdTe QDs-based prostate-specific antigen probe for human prostate cancer cell imaging |
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Authors: | Wei Dong Li Guo Shukun Xu |
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Affiliation: | a Department of Chemistry, Northeastern University, Shenyang 110004, People's Republic of China b Shenyang Medical College, Shenyang, Liaoning 110034, China |
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Abstract: | l-glutathione (GSH) stabilized CdTe quantum dots (QDs) were directly prepared in aqueous solution. The as-prepared QDs were linked to prostate-specific antigen (PSA) for the direct labeling and linked to immunoglobulin G (IgG) for the indirect labeling of fixed prostate cancer cells. The results indicated that QD-based probes were ideal fluorescent markers with excellent spectral properties and photostability and much better than organic dyes making them very suitable in target detection. Meanwhile, the indirect labeling showed much better specificity than the direct labeling. Furthermore, the prepared CdTe QDs did not show detectable effect on cell growth after having cultured for three days, which suggested that the l-glutathione capped CdTe had scarcely cytotoxicity. |
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Keywords: | Quantum dots CdTe Immuno-fluorescence Prostate cancer cell Direct labeling Indirect labeling |
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