2D NMR Studies on the Structure of Urushiol of Chinese Lacquer

The urushiol diacetate was successfully separated by centrifugal rotation TLCon the silica gel coated with silver nitrate.The structure and configuration of key component of.urushiol (64.6%) were determined successfully by using ¹H and ¹³C conventional method,DEPTtechnique,homo- and heteronuclear chemical shift correlation 2D-NMR,2D-J NMR,hftteronuclear relayed coherencetransfer 2D-NMR,and relaxation time T₁ investigation.The as-signment of resonances for ¹H and ¹³C were confirmed.There is plentyof evidence which shows that the key component of urushiol is 3-(8'Z,11'E,13'Z-pentadecatrienyl)catechol.The results show that RELAY 2D-NMR new technique can be successfully applied to determining the structure of complicated unknown compounds.