Determination of matrine in medicine and bio-fluids based on inhibition of luminol-myoglobin chemiluminescence |
| |
Authors: | Judong You Jiangman Liu Donghua Chen Zhenghua Song |
| |
Institution: | 1. Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science Northwest University, 710069, Xi’an, China
|
| |
Abstract: | A green, rapid and sensitive flow injection procedure based on the inhibition of the chemiluminescence (CL) intensity from the luminol-myoglobin (Mb) system is proposed for the determination of matrine. The decrement of CL signal was linear with the logarithm of the matrine concentration over the range from 10 to 1000 ng/mL (R 2 = 0.9978) offering a detection limit of 3.5 ng/mL. At a flow rate of 2.0 mL/min, one analysis cycle, including sampling and washing, could be accomplished in 0.5 min with a relative standard deviation (RSD) of less than 5.0%. The sensitive flow injection method was successfully applied to the determination of matrine in pharmaceutical injection and human serum, with recoveries from 94.1 to 113.4% and RSDs of less than 5.0%. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|