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Fluorescence Assay Based on Aptamer-Quantum Dot Binding to <Emphasis Type="Italic">Bacillus thuringiensis</Emphasis> Spores |
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| Authors: | Milada Ikanovic Walter E Rudzinski John G Bruno Amity Allman Maria P Carrillo Sulatha Dwarakanath Suneetha Bhahdigadi Poornima Rao Johnathan L Kiel Carrie J Andrews |
| Institution: | (1) Department of Chemistry and Biochemistry, Texas State University-San Marcos, 601 University Drive, San Marcos, TX 78666, USA;(2) Operational Technologies Corporation, 4100 NW Loop 410, Suite 230, San Antonio, TX 78229, USA;(3) Present address: Severn Trent Laboratories, 880 Riverside Pwky, West Sacramento, CA 95605, USA;(4) NanoScience Diagnostics, Inc., 1826 Kramer Lane, Suite E, Austin, TX 78758, USA;(5) Air Force Research Laboratory, HEPC, 2486 Gillingham Drive, Bldg. 175 E, Brooks City-Base, San Antonio, TX 78235, USA |
| Abstract: | A novel assay was developed for the detection of Bacillus thuringiensis (BT) spores. The assay is based on the fluorescence observed after binding an aptamer-quantum dot conjugate to BT spores. The in vitro selection and amplification technique called SELEX (Systematic Evolution of Ligands by EXponential enrichment) was used in order to identify the DNA aptamer sequence specific for BT. The 60 base aptamer was then coupled to fluorescent zinc sulfide-capped, cadmium selenide quantum dots (QD). The assay is semi-quantitative, specific and can detect BT at concentrations of about 1,000 colony forming units/ml. |
| Keywords: | Bacillus thuringiensis Aptamer Quantum dots SELEX Fluorescence |
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