Preparative separation of flavonoid glycosides in leaves extract of Ampelopsis grossedentata using high-speed counter-current chromatography |
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Authors: | Du Qizhen Chen Ping Jerz Gerold Winterhalter Peter |
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Affiliation: | Institute of Food and Biological Engineering, Hangzhou University of Commerce, Hangzhou 310035, China. qizhendu@mail.hzic.edu.cn |
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Abstract: | Preparative separation of flavonoid glycosides in leaves extract of Ampelopsis grossedentata was conducted using high-speed counter-current chromatograph (HSCCC) with a solvent system composed of n-hexane-ethyl acetate-methanol-water (1:6:1.5:7.5, v/v). In a single operation, 28 mg of 5,7-dihydroxy-3',4'-trihydroxyflavone-3-O-6'-rhamnose and 18 mg of 5,7-dihydroxy-3',4'-dihydroxyflavone-3-O-6'-rhamnose was obtained from 150 mg of the extract. The chemical structure of the two compounds was elucidated by electrospray ionization (EIS) MS and NMR. |
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Keywords: | Preparative chromatography Counter-current chromatography Plant materials Flavonoids Glycosides |
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