Analysis of Fenitrothion and Metabolites in Stored Wheat |
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| Authors: | M H K Abdel-kader G R B Webster |
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| Institution: | Pesticide Research Laboratory, Department of Soil Science , University of Manitoba , Winnipeg, Manitoba, Canada , R3T 2N2 |
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| Abstract: | Abstract A simple and rapid method for the analysis of fenitrothion and its metabolites, fenitrooxon, S-methyl fenitrothion, demethyl fenitrothion, demethyl S-methyl fenitrothion, 3-methyl-4-nitrophenol, and dimethyl phosphorothioic acid in stored wheat has been developed. Simultaneous analysis of the extract was conducted using FPD-GLC after derivatization with diazoethane except for 3-methyl-4-nitrophenol which was analyzed directly by EC-GLC. Recoveries of all compounds from wheat fortified at the levels from 0.1 to 5.0 ppm were greater than 90%. The developed method was used to quantitatively determine major metabolites found in grain treated with fenitrothion and stored at 20°C for 12 months. Demethyl fenitrothion, 3-methyl-4-nitrophenol, and dimethyl phosphorothioic acid were the major breakdown products of fenitrothion found in stored wheat. Confirmation of these metabolites was carried out by chemical derivatization plus FPD-GLC and by TLC. |
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| Keywords: | Laser-excited atomic fluorescence spectrometry thallium in situ known addition Great Lakes Hamilton Harbor direct determination clean room |
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