Chemiluminescent enzyme-linked immunosorbent assay on a strip to detect Escherichia coli O157:H7 |
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Authors: | Sojung Park Junhong Min |
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Institution: | 1. Division of Chemical Engineering , Hankyong National University , Anseong, Kyonggi-Do 456-749, Korea;2. Department of NanoBiotechnology , Kyungwon University, Seongnam , Kyonggi-Do 461-701 , Korea |
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Abstract: | A fast and sensitive chemiluminescent enzyme-linked immunosorbent assay method to measure pathogenic bacteria, Escherichia coli O157:H7, on immuno-chromatographic membrane was studied. Non-specific binding of proteins on membrane strip was controlled to attain the best performance of immunosensor by optimising the composition of a running buffer. The specificity of the proposed immunostrip was confirmed by conducting experiments for four different micro-organisms. A chemiluminescent signal could be successfully generated from a proposed immunostrip sensing system, and a significant change in the chemiluminescent light intensity with the concentration of target microbes was obtained. E. coli O157:H7 could be quantitatively measured in the range of 1.1?×?103?–1.1?×?107 CFU (colony forming units) mL?1 within 16?min by using the developed chemiluminescent immunostrip. |
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Keywords: | enzyme-linked immunosorbent assay (ELISA) chemiluminescence Escherichia coli O157:H7 chromatographic membrane strip immunosensor |
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