The Effects of a Strong Disaggregating Agent on Sec-Page of Aquatic and Soil Humic Matter |
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| Authors: | Juhani Peuravuori Kalevi Pihlaja Olga Trubetskaya Oleg Trubetskoj |
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| Affiliation: | 1. Department of Chemistry, Laboratory of Physical Chemistry , University of Turku , FIN-20014, Turku, Finland;2. Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry , 142292 Pushchino, Moscow region, Russia;3. Institute of Soil Science and Photosynthesis, Russian Academy of Sciences , 142292 Pushchino, Moscow region, Russia |
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| Abstract: | Abstract Size-exclusion chromatographic (SEC) fractionation and electrophoretic separation of aquatic humic matter samples from a Finnish lake using Sephadex G-75 with 7 M urea solution as eluent and 10% polyacrylamide gel (PAGE) with urea and sodium dodecyl sulphate solution (SDS), respectively, were performed and compared to similar analyses performed on a Russian chernozem soil humic acid sample and Nordic reference fulvic and humic acid samples. The integrated whole of aquatic humic solutes and soil humic acids were found to exhibit similar SEC-PAGE behaviours. Humic matter was not excessively disaggregated by the 7 M urea and hence SEC-PAGE can with confidence be applied as a coarse, initial fractionation procedure or for certain predeterminations of the structural composition. |
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| Keywords: | electrophoresis size-exclusion chromatography polyacrylamide gel humic matter chaotropic agent |
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