The Use of Automated Headspace Gas Chromatography for Determination of 1,1,1-Trichloroethane in Rat Blood and Brain Tissue |
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Authors: | Eva Westerberg Lennart Larsson |
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Affiliation: | 1. Laboratory for Experimental Brain Research , E-blocket, University Hospital, Lund;2. Institute of Technical Analytical Chemistry, and Medical Microbiology, University of Lund , Lund, Sweden |
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Abstract: | Abstract 1,1,1-trichloroethane in blood and brain tissue from rats which had been artificially ventilated with solvent (8000 ppm) was analysed by automated headspace gas chromatography using a fused silica capillary column. A given concentration of 1,1,1-trichloroethane in the brain could be correlated with a corresponding concentration in the blood; both the uptake and release of the solvent were quicker in blood than in brain. No volatile metabolites of the solvent were found. Automated headspace gas chromatographic analysis is a rapid and sensitive technique for the quantitative registration of volatile organic solvents, e.g. of industrial importance, in body fluids and tissues. |
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Keywords: | 1,1,1-trichloroethane headspace gas chromatography blood tissue. |
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