Determination of carbofuran by the quenching effect on resonance light scattering and its application to water and vegetable samples |
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| Authors: | Shicong Hou Yan Cui Fengpei Du Weifu Kong Junfen Song Ying Li |
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| Institution: | 1. Department of Applied Chemistry, College of Science , China Agricultural University , Beijing, P.R. China;2. Yantai Research Institute, China Agricultural University , Yantai, P.R. China |
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| Abstract: | A new method for the determination of carbofuran (CF) with DNA by a resonance light scattering (RLS) technique was developed. The intensity of RLS (I RLS) of DNA–HCl system was significantly quenched in presence of CF. A RLS peak at 315.6 nm was found, and the quenched intensity of RLS was proportional to the concentration of CF. The linear range of the calibration curve was ≈0.02–2.0 µg mL?1 and the detection limit (S/N = 3) 7 ng mL?1. The CF in river water, cucumbers and rice samples was determined. The recovery rates were in the range of 90.0–111.1, 95.0–106, 93.0–111.0%, respectively. The mechanism of the reaction between CF and DNA is also discussed. |
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| Keywords: | carbofuran resonance light-scattering method DNA quenching effect river water food samples |
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