牛血清白蛋白介导合成的金纳米簇用于活细胞荧光成像

以牛血清白蛋白介导合成金纳米簇, 并利用荧光分光光度计、纳米粒度及zeta电位仪以及非变性聚丙烯酰胺蛋白质电泳对其进行了表征. 结果表明, 该金纳米簇不仅荧光信号较强, 而且在不同pH值溶液中荧光稳定性好. 在此基础上进一步考察了金纳米簇与宫颈癌细胞(HeLa)间的相互作用. 结果表明, 该金纳米簇可成功进入活细胞内, 在最佳的培育时间和金纳米簇浓度条件下可达到较好的活细胞荧光标记效果, 且在经过细胞固定化处理后仍保持其标记形态.

Live Cells Fluorescence Imaging by Bovine Serum Albumin-mediated Synthesized Gold Nanoclusters

Gold nanocluster was synthesized by bovine serum albumin directing, and was characterized by fluorescence spectrophotometer, zeta sizer and non-denaturing polyacrylamide gel electrophoresis. The results show that the gold nanocluster’s fluorescence signal is strong and stable at different pH value. The interaction between gold nanoclusters and HeLa cells was further studied. It demonstrates that the gold naoclusters could be successfully uptaken by living cells. The good effects of the fluorescent labeling of living cells can be achieved by optimal incubation time and gold nanoclusters’ concentration. Moreover, the fixation treatment did not change the form of labeling. These results provide some guidance to the further application of fluorescent gold nanoclusters in the field of live cells labeling.