|
|||||
|
|
Liquid-solid extraction and HPLC determination of amiodarone metabolites in biological fluids |
|
| Authors: | M Petrarulo S Pellegrino E Mentasti |
| Institution: | (1) Laboratory of Renal Stone Disease, Ospedale Mauriziano Umberto I, L. go Turati 62, Torino, Italy;(2) Central Analysis Laboratory, Ospedale Mauriziano Umberto I, L. go Turati 62, Torino, Italy;(3) Department of Analytical Chemistry, University of Turin, Via Giuria 5, I-10125 Torino, Italy |
| Abstract: | Summary An improved liquid-solid extraction procedure for the purification and determination of amiodarone and desethylamiodarone in biological fluids is proposed. The sample is passed through a silica-C18 column, and after washing, the analytes are eluted with methanol. The determination is then accomplished by RP HPLC using an octadecyl silica column and a mobile phase of methanol containing 0.0015% of ammonium hydroxide. The effect of ammonia concentration on the capacity factors of the analytes has been used for estimating the acid dissociation constants of the investigated secondary and tertiary amines in methanol. |
| Keywords: | Column liquid chromatography Solid phase extraction Amiodarone in serum Addition of ammonia to eluent |
| 本文献已被 SpringerLink 等数据库收录！ | |