反相高效液相色谱法分离直链烷基苯磺酸钠同系物和异构体

建立了直链烷基苯磺酸钠的反相高效液相色谱分析方法。探讨了流动相中甲醇含量、电解质浓度及不同电解质对样品保留时间、选择性和分离度等的影响,得到最佳的色谱分离条件为：流动相为甲醇 30 mmol/L 磷酸二氢钠水溶液,采用折线梯度洗脱,检测波长为226 nm。结果表明,该法对烷基链为C8~C 16 的烷基苯磺酸钠的同系物能完全分离,对烷基苯磺酸钠的异构体有一定的分离能力。

Reversed-Phase High Performance Liquid Chromatographic Separation of Linear Alkylbenzenesulfonate Homologues and Positional Isomers

A reversed-phase high performance liquid chromatographic method has been developed. It is relatively simple and effective for the determination of linear alkylbenzenesulfonates (LAS). The effects of methanol content, electrolyte concentration and the type of electrolytes in mobile phase on the retention of LAS, selectivity, efficiency, and resolution are discussed. The optimal chromatographic conditions used were a reversed-phase C_18 column with detection wavelength at 226 nm and methanol-30 mmol/L sodium dihydrogen phosphate aqueous solution as mobile phase for gradient elution. A complete separation of the homologues has been obtained and the positional isomers were partially resolved.