RP-HPLC测定不同β-环糊精衍生物超分子包合物中褪黑激素的含量

建立了反相高效液相色谱法测定褪黑激素(MLT)与羟丙基-β-环糊精(HP-β-CD)、甲基-β-环糊精(M-β-CD)以及取代度为1、4、7的3种磺丁基醚-β-环糊精(SBE1-β-CD、SBE4-β-CD、SBE7-β-CD)共5种不同β-环糊精衍生物所形成的超分子包合物中褪黑激素含量的分析方法。采用Agilent Extend C18色谱柱(4.6mm×150mm,5μm),流动相为甲醇-水(40∶60),流速0.8mL/min,柱温:室温,检测波长278nm。结果表明:褪黑激素在5.05—202μg/mL浓度范围内线性关系良好(r=1.000);平均加标回收率分别为99.8%、99.0%、100.4%、99.9%、100.6%;RSD分别为0.53%、1.0%、0.71%、0.94%、0.86%。本方法可用于各类褪黑激素超分子包合物的含量分析。

Determination of Melatonin in Supramolecular Inclusion Complex of Different β-Cyclodextrin Derivatives by RP-HPLC

A RP-HPLC method for the quantitative determination of melatonin in five kinds of melatonin inclusion complex （MLT-HP-β-CD,MLT-M-β-CD,MLT-SBE1-β-CD,MLT-SBE4-β-CD,MLT-SBE7-β-CD） was established.The column was agilent extend C18（4.6mm×150mm,5μm）,the mobile phase was methanol-water （40：60）,and the detection wavelength was at 278nm.The flow rate was 0.8mL/min and the column temperature was at room temperature.The calibration curve of melatonin was linear in the concentration range of 5.05—202μg/mL（r=1.000）.The average recoveries were 99.8%,99.0%,100.4%,99.9%,100.6%;RSD were 0.53%,1.0%,0.71%,0.94%,0.86%.This method can be used for melatonin content analysis of various types of supramolecular inclusion complexes.