Vacuum UV laser induced scission of simian virus 40 DNA |
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| Authors: | M Johnson-Thompson J B Halpern W M Jackson J George |
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| Institution: | 1. ‡Department of Chemistry, Howard University, 525 College St. N. W., Washington. DC 20059.;2. §Department of Biochemistry, Georgetown University, Schools of Medicine and Dentistry, Washington, DC 2007, USA. |
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| Abstract: | Abstract— The effect of vacuum UV-laser irradiation on Simian virus 40 (SV40) DNA I was studied in vitro. Following exposure of SV40 DNA I to an argon fluoride (ArF) laser (LD = 193 nm), single-strand breaks occur in either strand of the double-stranded superhelical molecule (DNA I) and convert it to the DNA II conformation. Upon increased irradiation times, additional breaks occur in already scissioned strands resulting in the production of smaller fragments as analyzed by alkaline sucrose centrifugation. These results suggest that short periods of irradiation lead to conformational alterations of SV40 DNA I. |
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