A Modular Approach To Study Protein Adsorption on Surface Modified Hydroxyapatite |
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Authors: | Dr. Vinayaraj Ozhukil Kollath Freya Van den Broeck Dr. Krisztina Fehér Prof. José C. Martins Dr. Jan Luyten Dr. Karl Traina Dr. Steven Mullens Prof. Rudi Cloots |
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Affiliation: | 1. Department of Chemistry, University of Liège, B6a, Allée de?la?Chimie 3, Liège 4000 (Belgium);2. Sustainable Materials Management, Flemish Institute for Technological Research (VITO), Boeretang 200, Mol 2400 (Belgium);3. Present address: Department of Physics, UNESP, Univ. Estadual Paulista, 17033‐360, Bauru, SP (Brazil);4. Department of Organic Chemistry, Ghent University, Krijgslaan 281, Ghent 9000 (Belgium);5. Department of Metallurgy and Materials Engineering, Katholieke Universiteit Leuven, 3001 Heverlee (Belgium);6. APTIS, University of Liège, B5a, Allée de?la?Chimie 3, Liège 4000 (Belgium);7. Present address: Galephar MF, 6900 Marche en Famenne (Belgium) |
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Abstract: | Biocompatible inorganic nano‐ and microcarriers can be suitable candidates for protein delivery. This study demonstrates facile methods of functionalization by using nanoscale linker molecules to change the protein adsorption capacity of hydroxyapatite (HA) powder. The adsorption capacity of bovine serum albumin as a model protein has been studied with respect to the surface modifications. The selected linker molecules (lysine, arginine, and phosphoserine) can influence the adsorption capacity by changing the electrostatic nature of the HA surface. Qualitative and quantitative analyses of linker‐molecule interactions with the HA surface have been performed by using NMR spectroscopy, zeta‐potential measurements, X‐ray photoelectron spectroscopy, and thermogravimetric analyses. Additionally, correlations to theoretical isotherm models have been calculated with respect to Langmuir and Freundlich isotherms. Lysine and arginine increased the protein adsorption, whereas phosphoserine reduced the protein adsorption. The results show that the adsorption capacity can be controlled with different functionalization, depending on the protein–carrier selections under consideration. The scientific knowledge acquired from this study can be applied in various biotechnological applications that involve biomolecule–inorganic material interfaces. |
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Keywords: | adsorption amino acids NMR spectroscopy proteins surface chemistry |
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