Expanding the Scope of 2′‐SCF3 Modified RNA |
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Authors: | Lukas Jud Marija Ko?uti? Veronika Schwarz Dr Markus Hartl Dr Christoph Kreutz Prof?Dr Klaus Bister Prof?Dr Ronald Micura |
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Institution: | 1. Institute of Organic Chemistry, Center for Molecular Biosciences Innsbruck (CMBI), University of Innsbruck, Innrain 80–82, 6020 Innsbruck (Austria);2. Institute of Biochemistry, Center for Molecular Biosciences Innsbruck (CMBI), University of Innsbruck, Innrain 80–82, 6020 Innsbruck (Austria) |
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Abstract: | The 2′‐trifluoromethylthio (2′‐SCF3) modification endows ribonucleic acids with exceptional properties and has attracted considerable interest as a reporter group for NMR spectroscopic applications. However, only modified pyrimidine nucleosides have been generated so far. Here, the syntheses of 2′‐SCF3 adenosine and guanosine phosphoramidites of which the latter was obtained in highly efficient manner by an unconventional Boc‐protecting group strategy, are reported. RNA solid‐phase synthesis provided site‐specifically 2′‐SCF3‐modified oligoribonucleotides that were investigated intensively. Their excellent behavior in 19F NMR spectroscopic probing of RNA ligand binding was exemplified for a noncovalent small molecule–RNA interaction. Moreover, comparably to the 2′‐SCF3 pyrimidine nucleosides, the purine counterparts were also found to cause a significant thermodynamic destabilization when located in double helical regions. This property was considered beneficial for siRNA design under the aspect to minimize off‐target effects and their performance in silencing of the BASP1 gene was demonstrated. |
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Keywords: | fluorine NMR spectroscopy oligonucleotides phosphoramidites solid‐phase synthesis |
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