Bifunctional Diaminoterephthalate Scaffolds as Fluorescence Turn‐On Probes for Thiols |
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Authors: | Lena Freimuth Prof?Dr Jens Christoffers |
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Institution: | Institut für Chemie, Carl von Ossietzky Universit?t Oldenburg, 26111 Oldenburg (Germany), Fax: (+49)?441‐798‐3873 http://www.christoffers.chemie.uni‐oldenburg.de/17321.html |
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Abstract: | The fluorescent diaminoterephthalate scaffold was equipped by amidation with three types of reactive functions: thiols for metal‐surface binding, alkynes for click reactions, and maleimides for ligation with proteins. Starting from a succinyl succinate derivative with two orthogonally cleavable ester functions, three monoamides (38–57 % yield over three steps) and two bisamides (19 and 25 % yield over five steps) were prepared. Although alkyne and thiol derivatized compounds showed reasonable luminescence behavior (Φ≈1–4 %), the fluorescence was quenched by the maleimide moiety. It was turned on (10‐ to 20‐fold increase of fluorescence quantum yield) by conjugate addition of thiols. |
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Keywords: | alkynes fluorescence scaffolds terephthalates thiols |
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