Replacement of native copper in superoxide dismutase with copper-64 without much loss in its enzymatic activity |
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| Authors: | S Iqbal A Naqvi M H Rizvi |
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| Institution: | (1) Directorate of Nuclear Fuel and Materials, Pakistan Atomic Energy Commission, P. O. Box 1114, Islamabad, (Pakistan);(2) Nuclear Chemistry Division, Pakistan Institute of Nuclear Science and Technology, P. O. Nilore, Islamabad, (Pakistan) |
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| Abstract: | Superoxide dismutase, containing copper and zinc, has been labeled with copper-64 by incubating the prepared apoenzyme with cupric(65) chloride at room temperature. No significant loss in the enzymatic activity was observed after labeling. The incorporation of copper-64 was ascertained by starch gel electrophoresis and high performance liquid chromatography. The labeling efficiency was found to be >95%. |
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