新型荧光试剂1,5-二(4,6-二氯三嗪)-氨基萘与酪氨酸相互作用

合成了一种新型三嗪荧光探针1,5-二(4,6-二氯三嗪)-氨基萘并利用元素分析、IR和核磁共振谱进行了表征。在pH=12.0的柠檬酸钠-NaOH缓冲溶液中,与酪氨酸在35℃下反应30 min后,λex/λem=400 nm/465 nm处,1,5-二(4,6-二氯三嗪)-氨基萘与酪氨酸反应使体系的荧光强度增强,且增强的荧光强度与酪氨酸的浓度成正比,依此建立了一种测定酪氨酸的新方法。测定的线性范围是1.1×10-7~1.1×10-5mol/L,检出限为6.8×10-8mol/L。该方法成功用于测定人尿和血清样品中的酪氨酸。

Spectrofluorimetric Determination of Trace Amounts of Tyrosine with 1,5-Bis(4,6-dicholro-1,3,5-triazinylamino) naphthalene

1,5-Bis(4,6-dicholro-1,3,5-triazinylamino) naphthalene was synthesized as a completely new fluorescent reagent and with it a novel fluorescent method was developed for the determination of tyrosine. Under pH 12.0, the fluorescent intensity at λ_ex/λ_em = 400 nm/465 nm was directly proportional to the concentration of tyrosine in the range of 1.1 × 10⁻⁷–1.1 × 10⁻⁵ M with the detection limit of 6.8 ×10⁻⁸ M. The proposed method has been successfully applied to the determination of trace amounts of tyrosine in human serum and urine samples.