| 激光诱导荧光光度法测定食品中的锌 |
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| 引用本文： | 栾崇林,黎源倩,杨经国.激光诱导荧光光度法测定食品中的锌[J].光散射学报,2002,14(4):231-233. |
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| 作者姓名： | 栾崇林 黎源倩 杨经国 |
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| 作者单位： | 1. 四川大学华西公共卫生学院卫生检测教研室,610041
2. 四川大学物理系,610064 |
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| 基金项目： | 国家自然科学基金 (No .39770 6 46 )，国家教委博士点基金 (No .976 0 )资助课题 |
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| 摘 要： | 本文利用自行组装的激光诱导荧光 CCD阵列检测器 计算机联用装置 ,建立了一种测定食品中锌的灵敏方法。在乙酸 乙酸铵 ( pH =4 )缓冲溶液中 ,Zn(Ⅱ )与SCN、罗丹明B(RhB)反应生成Zn SCN RhB配合物 ,经乙醚萃取后 ,以YAG激光二倍频 5 32nm为激发波长 ,在 5 80nm处测定荧光强度 ,优化了反应及食品样品的处理条件 ,用于食品试样中锌的测定 ,获得了满意的结果 ,方法检出限 4ng/mL ,加标回收率90 0～ 1 1 0 0 % ,相对标准偏差为 2 0～ 9 7%。
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| 关 键 词： | 激光诱导荧光 CCD阵列检测器 锌 食品检验 |
| 文章编号： | 1004-5929(2002)04-0231-03 |
| 修稿时间： | 2001年7月30日 |
Determination of Zinc in Food by Laser Induced Fluorescence Spectroscopy |
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| LUAN Chong-lin,LI Yuan-qian ,YANG Jing-guo.Determination of Zinc in Food by Laser Induced Fluorescence Spectroscopy[J].Chinese Journal of Light Scattering,2002,14(4):231-233. |
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| Authors: | LUAN Chong-lin LI Yuan-qian YANG Jing-guo |
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| Institution: | LUAN Chong-lin1,LI Yuan-qian 1*,YANG Jing-guo2 |
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| Abstract: | A new sensitive method for determination of zinc in food by laser-induced fluorescence-CCD detector-computer has been developed. In sodium acetate buffer medium (pH=4), Zn(Ⅱ) reacts with SCN- and RhB to form a complex of Zn-SCN-RhB. The complex is extracted into ether and is measured at excitation wavelength of 532 nm and emission wavelength of 580 nm. The effect of several surfactants on the fluorescence intensity of complex of Zn-SCN-Rhb has been investigated. The conditions of the reaction and the pretreatment of food samples has been optimized. The limit detection of this method is 4 ng/ml. The spiked recoveries are in the range of 90.0%～110.0% and the relative standard deviation varies from 2.0% to 9.7%. The method is successfully applied to food samples for determining zinc in food. |
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| Keywords: | Laser-induced fluorescence CCD-diode array detector Zinc Fodd analysis |
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