羽衣甘蓝花药培养技术体系的研究

本实验以6份羽衣甘蓝为材料进行花药培养.细胞学观察表明,当花蕾长为2.5-3.5mm,花瓣与花药长度之比为1/2～4/5时,45-65%的小孢子发育处于单核晚期,是花药培养的最佳时期.花药培养结果可见基因型不同,胚状体的诱导率有极大的差异,最容易形成胚状体的材料是Y3,诱导效率达17.74%;胚状体的出现在花药接种后的15-30天之间.培养基的种类不同,培养效果也有差异,其中以Keller培养基效果最佳;培养基中有机成份加倍,则可以显著提高胚状体的诱导效率.培养基中蔗糖的浓度对胚状体的诱导率也有显著的影响.

Studies on the Technical System of AntherCulture of Brassica oleracea var. acephala

Six cultivars of Brassica oleracea var. acephala were used in the study anther culture. The main results are as follows: The cytological observations of pollen showed that most of the pollen were in the late uninucleate stage when the ratio of petal to anther length was between 1/2~4/5 in Brassica oleracea var. acephala ,and the stage of late uninucleate was optimal for anther culture. The effects of genotype and cultural medium on embryoid induction were investigated in s Brassica oleracea var. acephala. The results showed it was optimal to induce the embryoid in Keller medium with double organic ingredients and 10% sucrose. The capacity of embryoid production varied significantly in different genotypes, and genotype Y3 was an ideal material for embryoid induction .