| 水溶性CdTe量子点荧光猝灭法测定尼群地平的研究 |
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| 引用本文： | 贺冬秀,刘璐,喻翠云,何小珍. 水溶性CdTe量子点荧光猝灭法测定尼群地平的研究[J]. 分析测试学报, 2010, 29(12). DOI: 10.3969/j.issn.1004-4957.2010.12.007 |
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| 作者姓名： | 贺冬秀 刘璐 喻翠云 何小珍 |
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| 作者单位： | 南华大学,药学与生命科学技术学院,湖南,衡阳,421001;湖南师范大学,化学生物学及中药分析省部共建教育部重点实验室,湖南,长沙,410081;湖南师范大学,化学生物学及中药分析省部共建教育部重点实验室,湖南,长沙,410081;南华大学,药学与生命科学技术学院,湖南,衡阳,421001 |
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| 基金项目： | 国家自然科学基金资助项目,衡阳市科技计划资助项目 |
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| 摘 要： | 以巯基乙酸为稳定剂,直接合成了水溶性CdTe量子点。基于尼群地平对合成量子点的荧光猝灭效应,建立了一种简便、快速和灵敏地测定尼群地平的分析方法。考察了缓冲体系、缓冲液浓度、缓冲液pH值、反应时间、量子点浓度对尼群地平测定的影响,在0.03 mol/L、pH值为8.3的Tris-HCl缓冲液中,当量子点的浓度为6.0×10-4mol/L、反应时间为5 min,体系的相对荧光强度与尼群地平的质量浓度呈良好线性关系,其线性范围为1.09~65.4 mg/L,线性系数为0.998 6,检出限(S/N=3)为0.11 mg/L。该方法已成功用于药片中尼群地平的测定,与中国药典中的标准方法比较,结果满意。同时该文对尼群地平与CdTe量子点的反应机理进行了初步探讨。
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| 关 键 词： | CdTe 量子点 尼群地平 荧光猝灭 |
Study on Fluorescence Quenching Method for the Determination of Nitrendipine with Water-soluble CdTe Quantum Dots |
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| HE Dong-xiu,LIU Lu,YU Cui-yun,HE Xiao-zhen. Study on Fluorescence Quenching Method for the Determination of Nitrendipine with Water-soluble CdTe Quantum Dots[J]. Journal of Instrumental Analysis, 2010, 29(12). DOI: 10.3969/j.issn.1004-4957.2010.12.007 |
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| Authors: | HE Dong-xiu LIU Lu YU Cui-yun HE Xiao-zhen |
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| Affiliation: | HE Dong-xiu1,2,LIU Lu2,YU Cui-yun1,HE Xiao-zhen1(1.School of Pharmacy and Life Sciences,University of South China,Hengyang 421001,China,2.Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research(Hunan Normal University),Ministry of Education,Changsha 410081,China) |
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| Abstract: | Water-soluble CdTe quantum dots were synthesized in aqueous solution with thioglycolic acid as stabilizer.Based on the quenching of the fluorescence intensity of functionalized CdTe quantum dots(QDs) by nitrendipine,a simple,rapid and sensitive method for the determination of nitrendipine was proposed and validated.The effects of experimental conditions,including the type and pH of buffer,reacting time,concentration of CdTe QDs,on the determination of nitrendipine were investigated in detail.The optimal con... |
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| Keywords: | CdTe quantum dots nitrendipine fluorescence quenching |
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