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Chemical Structure and In Vitro Antitumor Activity of Rhamnolipids from Pseudomonas aeruginosa BN10 |
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| Authors: | Neli Christova Boryana Tuleva Anton Kril Milka Georgieva Spiro Konstantinov Ivan Terziyski Biliana Nikolova Ivanka Stoineva |
| Institution: | 1. Institute of Microbiology, Bulgarian Academy of Sciences, Acad. G. Bonchev str., bl 26, 1113, Sofia, Bulgaria 2. Institute for Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Acad. G. Bonchev str., bl 25, 1113, Sofia, Bulgaria 3. Laboratory of Molecular Diagnostics, Department of Pathology, National Oncological Centre, 6 Plovdivsko pole str., 1156, Sofia, Bulgaria 4. Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University–Sofia, 2 Dunav str., 1000, Sofia, Bulgaria 5. Institute of Physical Chemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev str., bl 11, 1113, Sofia, Bulgaria 6. Institute of Biophysics and Biomedical Engineering, Acad. G. Bonchev str., bl 21, 1113, Sofia, Bulgaria 7. Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev str., bl 9, 1113, Sofia, Bulgaria |
| Abstract: | A newly isolated indigenous strain BN10 identified as Pseudomonas aeruginosa was found to produce glycolipid (i.e., rhamnolipid-type) biosurfactants. Two representative rhamnolipidic fractions, RL-1 and RL-2, were separated on silica gel columns and their chemical structure was elucidated by a combination of nuclear magnetic resonance and mass spectroscopy. Subsequently, their cytotoxic effect on cancer cell lines HL-60, BV-173, SKW-3, and JMSU-1 was investigated. RL-1 was superior in terms of potency, causing 50 % inhibition of cellular viability at lower concentrations, as compared to RL-2. Furthermore, the results from fluorescent staining analysis demonstrated that RL-1 inhibited proliferation of BV-173 pre-B human leukemia cells by induction of apoptotic cell death. These findings suggest that RL-1 could be of potential for application in biomedicine as a new and promising therapeutic agent. |
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