| TWO EXPRESSED COPIES OF GLYCININ Gy4 GENE SUBFAMILY IN WILD SOYBEAN Glycine soja SHI |
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| 作者姓名： | 薛中天 徐美琳 庄乃亮 沈纬 沈善炯 |
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| 作者单位： | Laboratory of Molecular Genetics，Shanghai Institute of Plant Physiology，Academia Sinica，Laboratory of Molecular Genetics，Shanghai Institute of Plant Physiology，Academia Sinica，Laboratory of Molecular Genetics，Shanghai Institute of Plant Physiology，Academia Sinica，Laboratory of Molecular Genetics and Biotechnology，Jiaotong University，Shanghai，Laboratory of Molecular Genetics，Shanghai Institute of Plant Physiology，Academia Sinica |
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| 基金项目： | Project supported by the National Natural Science Foundation of China. |
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| 摘 要： | To study the structure of genes encoding seed storage proteins of the wild variety of soybean (Glycine soja) in comparison with those of the cultivated (Glycine max),cDNA libraries from cotyledon poly(A)~+ RNA of both wild and cultivated soybeans were constructed.Six clones were isolated using the genomic clone λS312 which contains the coding sequence for the glycinin subunit precursor (A_5A_4B_3) as probe for molecular hybridization. Two clones pWS228 and pWS242 contain the full length of cDNA.Restriction mapping of the cloned cDNA and Southern hybridization of the cDNA with genomic DNA showed two expressed copies of glycinin Gy4 gene subfamily in wild soybean Glycine soja SHI. Alteration of DNA arrangement in group-Ⅱ glycinin gene subfamily during embryogenesis was also demonstrated.
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