A Simple Procedure for Decomposition of Human Hair Using Polypropylene Vials for Selenium Determination by Hydride Generation Atomic Absorption Spectrometry |
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Authors: | Sergio R Mortari Ana Paula F Saidelles Juliano S Barin Érico M M Flores Ayrton F Martins |
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Institution: | (1) Departamento de Química, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil;(2) Área de Ciências da Saúde, Centro Universitário Franciscano, 97010-032 Santa Maria, RS, Brazil |
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Abstract: | Two procedures for wet decomposition of human hair samples were compared for selenium determination by hydride generation atomic absorption spectrometry (HGAAS). These procedures involve treatment with HNO3/H2O2 and H2SO4/HNO3, respectively, using small disposable polypropylene (PP) vials. Analytical steps from weighing to dilution were performed in the same individual PP vial. After sample weighing (10 ± 3 mg) and reagent addition, decomposition was performed in a conventional oven (20 h, 100 °C). Addition of sulfamic acid prior to HGAAS measurements was necessary to minimize interference due to residual nitrogen oxides from the decomposition step. The accuracy was estimated using certified hair reference analysis which yielded an agreement higher than 97% (recovery tests). The absolute limit of detection was 1.2 ng (3 s; n = 10), corresponding to 0.12 µg g–1 in the dry sample. |
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Keywords: | : Selenium determination HGAAS hair decomposition PP vials digestion |
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