Opto-electrochemical nanosensor array for remote DNA detection |
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Authors: | Deiss Frédérique Laurent Sébastien Descamps Emeline Livache Thierry Sojic Neso |
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Affiliation: | Groupe Nanosystèmes Analytiques, Institut des Sciences Moléculaires, CNRS UMR 5255, Université Bordeaux 1, ENSCPB, 16 avenue Pey-Berland, 33607 Pessac, France. |
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Abstract: | A high-density array of opto-electrochemical nanosensors is presented for remote DNA detection. It was fabricated by chemical etching of a coherent optical fibre bundle to produce a nanotip array. The surface of the etched bundle was sputter-coated with a thin ITO layer which was eventually insulated by an electrophoretic paint. The fabrication steps produced a high-density array of electrochemical nanosensors which retains the optical fibre bundle architecture and its imaging properties. A DNA probe was then immobilized on the nanosensor array surface in a polypyrrole film by electropolymerisation. After hybridisation with the complementary sequence, detection of the strepavidin-R-phycoerythrin label is performed by fluorescence imaging through the optical fibre bundle itself. Control experiments and regeneration steps have also been successfully demonstrated on this nanostructured opto-electrochemical platform. |
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