嵌合异硫氰酸罗丹明B核壳荧光纳米颗粒制备的新方法研究

随着纳米技术的发展，结合了纳米技术与材料制备技术而发展起来的荧光染料嵌合的核壳荧光纳米颗粒的制备为生物医学领域的研究提供了新的材料、技术和方法。何晓晓等以联钉吡啶配合物为核材料，制备了嵌合无机金属配合物的核壳荧光纳米颗粒，段菁华等用异硫氰酸荧光素FITC与蛋白质IgG相结合，

New Method for Preparing Core-shell Fluorescent Nanoparticles Doped with Rhodamine B Isothiocyanate

In this article, the preparation of core-shell fluorescent dye nanoparticles doped with rhodamine B isothiocyanate by using novel method is reported, which involves synchronous hydrolysis of tetraethoxysilane(TEOS) in water in oil microemulsion with the combination of N-(β-amimoethyl)-γ-amino propyltriethoxy-silane(AEAPS) and rhodamine B isothiocyanate(TRITC). This method conquers the disadvantages of traditional preparation method about organic dye doped core-shell nanoparticles such as the leakage of fluorescent dyes and the low encapsulation efficiency of fluorescent dyes. The fluorescent nanoparticle prepared by using the novel method have the advantages such as high fluorescence and good fluorescent stability, which are hopeful to be applied in the field of biological labelling.