| 补体C1q压电免疫传感器的研究 |
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| 引用本文： | 刘栗加,胡继明,裴仁军,沈竞凯.补体C1q压电免疫传感器的研究[J].分析化学,1999,27(10):1190-1192. |
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| 作者姓名： | 刘栗加 胡继明 裴仁军 沈竞凯 |
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| 作者单位： | 武汉大学分析测试科学系,武汉,430072 |
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| 基金项目： | 国家自然科学基金;39670218; |
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| 摘 要： | 研制了一种可重复使用的压电免疫传感器用于检测补体C1q.比较了聚乙烯亚胺粘附、戊二醛交联法,物理吸附法以及蛋白A法三种固定化抗体蛋白的方法.使用蛋白A法,补体C1q在所测浓度范围内有良好的线性关系.使用过的晶片用0.1 mol/L柠檬酸盐缓冲溶液(pH 3.0)解吸,可重复使用4次.
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| 关 键 词： | 压电免疫传感器 抗体的定向固定 补体 |
| 修稿时间： | 1998年11月23日 |
Piezoelectric Immunosensor for the Detection of Complement C1q |
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| Liu Lijia,Hu Jiming,PEI Renjun,Shen Jingkai.Piezoelectric Immunosensor for the Detection of Complement C1q[J].Chinese Journal of Analytical Chemistry,1999,27(10):1190-1192. |
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| Authors: | Liu Lijia Hu Jiming PEI Renjun Shen Jingkai |
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| Abstract: | A reusable piezoelectric immunosensor has been developed for the detection of human complement C1q. Three methods for immobilization of anti-C1q antibody on the gold electrodes were employed. The protein A method gave the best results in terms of sensitivity, stability and reproducibility. With the protein A method, the coated crystal had a good response in relevant concentration range. The electrodes could be reused 4 times by desorption of the immunocomplex with 0.1 mol/L citrate buffer. |
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| Keywords: | Piezoelectric immunosensor oriented immobilization of antibody complement C1q |
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