Individual particle handling in a microfluidic system based on parallel laser trapping |
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Authors: | Hamel Philippe Rachet Bastien Werner Michael Grossenbacher Mathieu Vogel Horst Forrer Martin Ryser Peter Salathé René P |
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Institution: | Applied Optics Laboratory, School of Engineering, EPFL, Station 17, CH-1015 Lausanne, Switzerland. philippe.hamel@epfl.ch |
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Abstract: | We present an optical trapping system combining individually addressable multiple laser traps with fluorescence spectroscopy. An in-line set of 64 near-IR laser diodes is used to create a line of individually addressable traps inside a microfluidic chip. This system is completed by an excitation/detection line for spectrally resolved fluorescence imaging of trapped particles. Highly parallel trapping in a constant flow (up to a few millimeters per second), fast particle handling rates (up to a few particles per second), and the possibility of recording fluorescence spectra of trapped objects lead to a performing bioanalytical platform, e.g., for highly parallel analysis and sorting. |
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