| 薄层扫描快速检测发酵液中琥珀酸 |
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| 引用本文： | 林日辉,余运玲,黄文勤,门媛媛. 薄层扫描快速检测发酵液中琥珀酸[J]. 分析试验室, 2012, 31(2): 59-61 |
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| 作者姓名： | 林日辉 余运玲 黄文勤 门媛媛 |
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| 作者单位： | 1. 广西民族大学化学与生态工程学院,化学与生物转化过程新技术广西高校重点实验室,南宁530006
2. 南宁奕德环境科技有限公司,南宁,530003 |
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| 基金项目： | 国家民委科研项目，广西科学基金，广西教育厅科研项目 |
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| 摘 要： | 基于CuSO4与发酵液中琥珀酸的显色反应,在硅胶板上不经层析展开使用薄层扫描定量分析琥珀酸含量.在0～12 g/L范围内,测定波长680 nm,参比波长500 nm条件下,琥珀酸浓度与显色斑吸光度呈良好线性关系.方法检出限(3 S/k)为0.028 mg/L,回收率94.80％～96.12％,相对标准偏差≤3.5％(n...
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| 关 键 词： | 发酵 琥珀酸 检测 薄层扫描 |
Determination of succinic acid in fermentation broth using thin layer scanning technique |
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| LIN Ri-hui , YU Yun-ling , HUANG Wen-qin , MEN Yuan-yuan. Determination of succinic acid in fermentation broth using thin layer scanning technique[J]. Chinese Journal of Analysis Laboratory, 2012, 31(2): 59-61 |
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| Authors: | LIN Ri-hui YU Yun-ling HUANG Wen-qin MEN Yuan-yuan |
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| Affiliation: | 1(1.Key Laboratory of New Techniques for Chemical and Biological Conversion Process,College of Chemistry and Ecological Engineering,Guangxi University for Nationalities,Nanning 530006;2.Nanning YiDe Environment Technology Limited Company,Nanning 530003) |
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| Abstract: | Based on color reaction between copper sulfate and succinic acid in fermentation broth,succinic acid concentration was determined directly by using thin layer scanning without chromatography.Linear relationship between the value of absorbance and the succinic acid concentration was obtained in the range of 0-12 g/L,using 680 nm as measurement wavelength and 500 nm as reference wavelength for scanning.The method detection limit(3 S/k) was 0.028 mg/L with the recoveries of 94.8%～96.1%,and the RSD(n=5) was lower than 3.5%.The interference rate of byproducts was lower than 5.60%.The paper provided an easy but efficient method for the determination of succinic acid in lab study or industry production. |
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| Keywords: | Fermentation Succinic acid Determination Thin layer scanning |
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