| 邻硝基苯基荧光酮与头孢唑啉的荷移反应研究 |
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| 引用本文： | 安娜,敖登高娃,于彦珠. 邻硝基苯基荧光酮与头孢唑啉的荷移反应研究[J]. 分析试验室, 2012, 31(1): 102-104 |
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| 作者姓名： | 安娜 敖登高娃 于彦珠 |
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| 作者单位： | 内蒙古大学化学化工学院,呼和浩特,010021 |
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| 基金项目： | 内蒙古自然科学基金,内蒙古大学创新人才培养项目 |
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| 摘 要： | 采用分光光度法,以邻硝基苯基荧光酮(O-NPF)为显色剂,研究了O-NPF与头孢唑啉(CFZ)络合物的可见光谱性质及测定条件。结果表明:CFZ在4.545～72.72μg/mL范围内遵循比尔定律,回归方程为:A=0.668ρ+0.5341,检出限1.53μg/mL,表观摩尔吸光系数为5.41×104L.mol-1.cm-1,相关系数为R2=0.9891,并探讨了其反应机理。方法已用于尿样及生物样品中CFZ含量的测定。
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| 关 键 词： | 邻硝基苯基荧光酮 头孢唑啉 分光光度法 |
Study on charge-transfer reaction between o-nitro phenyl fluorone and cefazolin |
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| AN Na , AODENG Gawa , YU Yan-zhu. Study on charge-transfer reaction between o-nitro phenyl fluorone and cefazolin[J]. Chinese Journal of Analysis Laboratory, 2012, 31(1): 102-104 |
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| Authors: | AN Na AODENG Gawa YU Yan-zhu |
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| Affiliation: | AN Na,AODENG Gawa* and YU Yan-zhu(School of Chemistry and Chemical Engineering,Inner Mongolin University,Hohholt 010021) |
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| Abstract: | The visible spectral characterist ic and the reaction condition of the complex of o-nitro phenyl fluorone(O-NPF) and cefazolin(CFZ) were investigated by spectrophotometry with O-NPF as a developer.The results showed that the concentration of CFZ obeyed the Beer’s law in the range of 4.545～72.72μg /mL and the regression equation was A=0.668ρ+0.5341 with the correlation coefficient r of 0.9891,the apparent molar absorptivity was 5.41×104 L·moL-1·cm-1and the detection limit was 1.53μg /mL.Further more,the reaction mechanism had been explored.The proposed method has been successfully applied to the determination of CFZ in urine and biological samples,and the results were satisfying. |
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| Keywords: | Cefazolin o-nitro phenyl fluorone Spectrophotometry |
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