| 甲基紫与牛血清白蛋白作用的荧光光谱研究 |
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| 引用本文： | 尹爱萍,白佛,李慧卿. 甲基紫与牛血清白蛋白作用的荧光光谱研究[J]. 分析试验室, 2012, 31(2): 71-74 |
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| 作者姓名： | 尹爱萍 白佛 李慧卿 |
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| 作者单位： | 忻州师范学院化学系,忻州,034000 |
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| 基金项目： | 山西省高等学校科技研究开发项目，忻州师范学院科研基金 |
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| 摘 要： | 在pH 7.40的Tris-HC1缓冲溶液体系中,采用荧光光谱法和同步荧光光谱法研究了甲基紫(MV)与牛血清白蛋白(BSA)相互作用.结果表明MV与BSA相互作用两者存在一个结合位点,结合常数(KA)为7.628×103 L/mol,MV与BSA主要发生疏水作用,反应是一个吸热、熵增的自发过程.
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| 关 键 词： | 甲基紫 牛血清白蛋白 荧光 |
Study on the interaction of bovine serum albumin with methyl violet by fluorescence spectroscopy |
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| YIN Ai-ping , BAI Fo , LI Hui-qing. Study on the interaction of bovine serum albumin with methyl violet by fluorescence spectroscopy[J]. Chinese Journal of Analysis Laboratory, 2012, 31(2): 71-74 |
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| Authors: | YIN Ai-ping BAI Fo LI Hui-qing |
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| Affiliation: | (Department of Chemistry Xin zhou Normal University,xinzhou 034000) |
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| Abstract: | The binding reaction between methyl violet and bovine serum albumin was studied by fluorescence spectroscopy in the Tris-HCl buffer system(pH=7.40).The result shows that methyl violet and bovine serum albumin has one binding site.Association constant is 7.628×103 L/mol,the interaction between bovine serum albumin and methyl violet is hydrophobic interaction,and the reaction is a process of heat absorption and entropy increase. |
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| Keywords: | Methyl violet Bovine Serum albumin Fluorescence |
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