原代大鼠肝细胞在多孔壳聚糖及其复合物支架上的培养

研究了原代肝细胞在壳聚糖及其复合物多孔支架上的生长及代谢.结果表明,细胞在多孔壳聚糖支架上生长良好,且密度、代谢活性较单层培养条件下有大幅度提高,细胞在7d后仍能保持较强的分泌白蛋白和合成尿素的功能,壳聚糖复合物上肝细胞的代谢活性更高.还从材料的化学结构和几何形态角度对这种材料的优势进行了讨论.

Culture of Primary Rat Hepatocytes on Porous Chitosan Scaffolds

Chitosan is considered to be a very promising biopolymer for various biomedical uses because of its nontoxic and biocompatible properties. In this study, porous chitosan scaffolds were prepared by freezing and lyophilization of chitosan gel. Scaffolds were modified with water-soluble polyanionic alginate. These scaffolds were viewed via scanning electron microscopy. The scaffolds with mean pore sizes from 50 to 200 μm had a high porosity of about 90%. Because of their large surface-to-volume ratio and the three-dimensional structure, they were used as the substrate for hepatocytes culture. The cell attachment ratio was much higher than on monolayer membrane. On the porous scaffolds, hepatocytes exhibited a round cellular morphology with many microvilli evidences on the surfaces indicating healthy cells. Metabolic activities were evaluated in terms of albumin secretion and urea synthesis. It was found that hepatocytes cultured on the modified scaffolds showed an increase in albumin secretion in the first 4 days and exhibited more stable albumin secretion than those on monolayer membrane and non-modified scaffold. Therefore, the primary rat hepatocytes culture using these modified scaffolds would be beneficial to liver assist device.