| 桥序列在构建人工核酶M1GS中的作用 |
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| 引用本文： | 郑永霞,李弘剑,李月琴,陈浩军,唐冬生,张欣,周天鸿.桥序列在构建人工核酶M1GS中的作用[J].暨南大学学报,2005,26(2):151-155. |
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| 作者姓名： | 郑永霞 李弘剑 李月琴 陈浩军 唐冬生 张欣 周天鸿 |
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| 作者单位： | 暨南大学生物工程学系,广东,广州,510632;暨南大学生物工程学系,广东,广州,510632;暨南大学生物工程学系,广东,广州,510632;暨南大学生物工程学系,广东,广州,510632;暨南大学生物工程学系,广东,广州,510632;暨南大学生物工程学系,广东,广州,510632;暨南大学生物工程学系,广东,广州,510632 |
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| 基金项目： | 国家自然科学基金 (30 370 776 )，广东省自然科学基金重大项目 (36 70 3)，广东省自然科学基金 (0 2 116 2，0 0 0 718)资助项目 |
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| 摘 要： | 目的：为检验连接的Gs序列是否会影响M1RNA高级结构而导致M1RNA活性改变，对核酶的催化机制进行探讨。方法：通过软件模拟对M1GS进行结构分析，筛选了一段独立折叠的桥序列连接M1RNA与GS，并通过体外切割实验检验有桥和无桥序列的核酶的活性。结果：有桥序列的M1GS具备体外特异切割底物的核酶活性，而无桥序列的M1GS核酶未表现体外切割活性。结论：证实桥序列对维持M1RNA高级结构和保持人工核酶M1GS的体外切割活性具有重要作用。
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| 关 键 词： | 核酶 核酶 P 人巨细胞病毒 UL54基因 |
| 文章编号： | 1000-9965(2005)02-0151-05 |
| 修稿时间： | 2004年10月9日 |
The role of bridge sequence in the construction of artificial ribozyme M1GS |
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| ZHENG Yong-xia,LI Hong-jian,LI Yue-qin,CHEN Hao-jun,TANG Dong-sheng,ZHANG Xin,ZHOU Tian-hong.The role of bridge sequence in the construction of artificial ribozyme M1GS[J].Journal of Jinan University(Natural Science & Medicine Edition),2005,26(2):151-155. |
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| Authors: | ZHENG Yong-xia LI Hong-jian LI Yue-qin CHEN Hao-jun TANG Dong-sheng ZHANG Xin ZHOU Tian-hong |
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| Abstract: | Aim: To elucidate the influence of covalently GS on the conformation and function of M1RNA in M1GS. Methods: Applying the RNA second structure software to simulate structure of M1GS, a sequence folded independently as bridge between M1RNA and GS was screen out. The enzyme activities of M1GS with bridge and M1GS without bridge were tested in vitro. Results: The M1GS with bridge can efficiently cleave the substrate, UL54 RNA fragment, however, the M1GS without bridge don't show activity of cleavage. Conclusion: It confirm that the bridge contribute to the completeness of confirmation of M1RNA, and play an important role in keeping the M1GS' activity of in vitro cleavage. |
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| Keywords: | ribozyme RNase P HCMV UL54 gene |
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