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| 聚吡咯为基质的脲酶传感器生物电化学响应 | |
| 引用本文： | 程发良,莫金垣,戴晓云.聚吡咯为基质的脲酶传感器生物电化学响应[J].高分子学报,1999,21(4):417-421. |
| 作者姓名： | 程发良 莫金垣 戴晓云 |
| 作者单位： | 1. 东莞理工学院应用化学系,东莞,511700 2. 中山大学化学系,广州,510275 3. 香港科技大学生化系,香港 |
| 摘 要： | 用电化学方法在吡咯（Ｐｙ）聚合的同时，将脲酶掺杂进聚吡咯膜（ＰＰｙ）中形成以聚吡咯为基质的脲酶电极．将脲酶电极与ＣＯ２气敏电极结合，组成对脲有电位响应的脲酶生物传感器．测试了传感器对脲的生物电化学响应．研究了聚合条件对酶活性的影响，并测定了掺杂于聚吡咯中的脲酶的活化能等动力学参数．在５０×１０－５～１．０×１０－２ｍｏｌ／Ｌ的浓度范围内，传感器响应的电极电位与脲浓度的对数成正比． |
| 关 键 词： | 脲酶，脲，聚吡咯，酶电极，生物传感器，气敏电极 |
BIOELECTROCHEMICAL RESPONSE OF POLYPYRROLE MODIFIED UREASE BIOSENSOR |
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| CHENG Faliang,MO Jingyuan,DAI Xiaoyun.BIOELECTROCHEMICAL RESPONSE OF POLYPYRROLE MODIFIED UREASE BIOSENSOR[J].Acta Polymerica Sinica,1999,21(4):417-421. | |
| Authors: | CHENG Faliang MO Jingyuan DAI Xiaoyun |
| Abstract: | Urease was doped into polypyrrole (PPy) film when pyrrole (Py) was polymerized by cyclic voltammogram. By Combination of PPy modified urease electrode and CO\-2 gas\|sensitive electrode a bioelectrochemical sensor was formed, which responses to urea. Factors affecting the activity of urease in PPy film were studied and activation energy of the ferment was determined. It was found that electrode potential is in direct proportion to the logarithm of the concentration of urea within the concentration range of 5.010-51.010-2mol/L.\= |
| Keywords: | Urea Urease Polypyrrole Biosensor |
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