Development and validation of a qualitative screening method for the detection of exogenous anabolic steroids in urine by liquid chromatography-tandem mass spectrometry |
| |
Authors: | Oscar J Pozo Peter Van Eenoo Koen Deventer Frans T Delbeke |
| |
Institution: | (1) DoCoLab, Department of Clinical Chemistry, Microbiology and Immunology, University of Ghent, Technologiepark 30, 9052 Zwijnaarde, Belgium;(2) Research Institute for Pesticides and Water, University Jaume I, 12071 Castellón, Spain |
| |
Abstract: | A screening method for the urinary detection of 34 exogenous anabolic steroids has been developed. The method involves an
enzymatic hydrolysis, liquid-liquid extraction and detection by liquid chromatography-tandem mass spectrometry. The use of
some adducts such as M+NH4]+, M+CH3COO]− and M+H+MeOH]+ was necessary in order to detect some analytes at the required level (lower than 10 ng/ml). Two transitions were selected
for each analyte. Different concentration factors have been studied in order to increase the sensitivity. A concentration
factor of 50 was selected for the screening method although the high ion suppression observed under these conditions can hamper
its application as a quantitative method. The method was validated and limits of detection were obtained by spiking ten different
blank urine samples at five different concentration levels. Up to 29 analytes were detected in all spiked urines at the required
level. Limits of detection between 1 and 10 ng/ml were obtained for most analytes which fulfil current requirements. The applicability
of the method was shown by analysing positive samples. |
| |
Keywords: | Anabolic steroids Screening Doping analysis Metabolites Mass spectrometry |
本文献已被 PubMed SpringerLink 等数据库收录! |
|