Individual and simultaneous fluorimetric determination of glycine and cysteine by flow injection analysis |
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Authors: | Baldomero Bermúdez Fernando Lzaro M Dolores Luque de Castro Miguel Valcrcel |
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Institution: | Baldomero Bermúdez, Fernando Lázaro, M. Dolores Luque de Castro,Miguel Valcárcel |
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Abstract: | A dual-channel flow injection configuration is proposed for individual and simultaneous determinations of glycine and cysteine (based on their reaction with phthaldialdehyde) in the range 1 × 10−7 – 1.5 × 10−5 M, with relative standard deviations of ± 1.7 and ± 1.3%, respectively, and a sampling frequency of 75–80 hr−1 for both analytes. The inclusion of a selecting valve in the configuration allows the simultaneous determination of these analytes in the concentration range achieved in their individual determinations. The resolution of mixtures is feasible with average errors of ± 2.2%. |
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