Reagentless enzymatic sensors based on carbon-paste electrodes containing ruthenium mediators for the on-line determination of glycerol |
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| Authors: | E V Ivanova W Schuhmann A D Ryabov |
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| Institution: | (1) Department of Chemistry, Moscow State University, Moscow, 119992, Russia;(2) Department of Chemistry, Ruhr University, Bochum, D-44780, Germany;(3) Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburg, PA 15213, USA |
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| Abstract: | A new design is proposed for biosensors based on carbon-paste electrodes enriched with ruthenium-containing acyclic compounds. Two biologically sensitive elements, a fragment of the cellular wall of Gluconobacter sp.33 containing a new enzyme PQQ-dependent glycerol dehydrogenase and a commercially available preparation of NAD-dependent glycerol dehydrogenase, are analyzed and compared. The activity of biologically sensitive preparations is studied using artificial electron carriers represented by ruthenium-containing acyclic and coordination compounds. |
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