Horizontal two-dimensional electrophoresis with immobilized pH gradients using PhastSystem |
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| Authors: | A G?rg W Postel S Günther C Friedrich |
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| Affiliation: | Lehrstuhl für Allgemeine Lebensmitteltechnologie, Technische Universit?t München, Freising-Weihenstephan, Federal Republic of Germany. |
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| Abstract: | Protocols for horizontal two-dimensional electrophoresis with immobilized pH gradients in the first dimension were modified for horizontal micro two-dimensional electrophoresis using PhastSystem. Different equilibration conditions of the first-dimensional immobilized pH gradient gel strip prior to second-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis were evaluated. Silver stained two-dimensional patterns were obtained within 3.5 h. |
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