碱性藏花红-十二烷基苯磺酸钠-蛋白质体系的荧光反应及其分析应用

阳离子染料碱性藏花红(ST)及其二聚体可平衡转化，形成平衡体系。在适宜浓度的阴离子表面活性剂的存在下，蛋白质的定量加入可调节这一平衡，引起体系荧光有规律的变化。在此基础上，研究了体系的吸收光谱和荧光光谱特性，建立了一种测定蛋白质的方法。实验表明，碱性藏花红在阴离子表面活性剂的作用下，自聚程度增加，体系荧光降低。加入蛋白质体系荧光增强，且增强程度与体系中的蛋白质的加入量呈线性关系。在优化实验条件下，线性范围为0～40 μg·mL-1,检出限为0.034 μg·mL-1。方法简便、快速、准确、灵敏度高，选择性好，用于人血清蛋白的测定，结果令人满意。

Fluorescence Reaction of the System of Safranine T-Sodium Dodecylbenzene Sulfonate-Protein and Its Application

There is a self-polymerization equilibrium system between the cation of the fluorescent dye-safranine T and its dimer. In the presence of appropriate amounts of anionic surfactant SDBS, the above mentioned equilibrium can be adjusted by a quantitative addition of protein, thus leading to a regular change in the fluorescence intensity of the system. Based on this phenomenon, the properties of the absorption spectrum and fluorescence spectrum of the system were studied, and a new fluorescence probe for the determination of protein was established. It was found that ST was apt to dimerize in the solution added with appropriate amounts of anionic surfactant. Because of the dimerization, its fluorescence intensity decreased, whereas the addition of protein caused the dimer to be depolymerized and the system fluorescence increased. A linear relationship between the fluorescence intensities and serum albumin concentration was found in the range of 0-40 microg x mL(-1) , and the detection limit found was 0.034 microg x mL(-1). The significant features of this method were its rapidity of reaction, and high sensitivity, accuracy and selectivity. The method was applied to the determination of total proteins in real human serum, and satisfactory results were obtained.