Polystyrene resins cross-linked with di- or tri(ethylene glycol) dimethacrylates as supports for solid-phase peptide synthesis |
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Authors: | Yu Wang Husheng Yan Yunge Fan Yanling Lu Wenhua Jiang Suwei Li |
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Institution: | a Key Laboratory of Functional Polymer Materials, Ministry of Education of China, Institute of Polymer Chemistry, Nankai University, Tianjin 300071, China b Tianjin Nankai Hecheng Science and Technology Co. Ltd, 94 Weijin Road, Tianjin 300071, China |
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Abstract: | Polystyrene resins cross-linked with di(ethylene glycol) dimethacrylate (DEGDMA) and tri(ethylene glycol) dimethacrylate (TEGDMA), DEGDMA-PS and TEGDMA-PS, were synthesized by suspension copolymerization. Four functionalized resins, chloromethyl resin, 4-hydroxymethylphenoxymethyl resin (Wang resin), 4-methylbenzhydrylamine resin (MBHA resin) and 2-chlorotrityl chloride resin, were prepared from DEGDMA-PS and TEGDMA-PS. DEGDMA-PS and TEGDMA-PS showed high reactivity in the functionalization reactions in comparison with Merrifield resin (polystyrene cross-linked with divinylbenzene, DVB-PS). DEGDMA-PS-Wang resin and TEGDMA-PS-Wang resin were used as the solid-phase support for the synthesis of a difficult sequence, the fragment of acyl carrier protein 65-74. The yields of the crude peptide synthesized using DEGDMA-PS-Wang resin, TEGDMA-PS-Wang resin and DVB-PS-Wang resin were 92.3%, 91.6% and 78.8%, respectively. The purities of the crude peptides were 85.7%, 88.1% and 73.3%, respectively. |
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Keywords: | Solid-phase peptide synthesis Di(ethylene glycol) dimethacrylate Tri(ethylene glycol) dimethacrylate Solid support |
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