Efficient synthesis of antisense phosphorothioate oligonucleotides using a universal solid support |
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Authors: | R Krishna Kumar |
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Institution: | Isis Pharmaceuticals, 2282 Faraday Avenue, Carlsbad, CA 92008, USA |
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Abstract: | It is demonstrated that solid support containing a novel universal linker could be efficiently used to synthesize both phosphorothioate oligodeoxyribonucleotides and second-generation 2′-O-methoxyethyloligoribonucleotides with high yield and quality as judged by ion-pair-liquid chromatography-electrospray mass spectroscopy, 31P NMR and reversed phase HPLC. Analysis of oligonucleotides shows quality being superior to that produced with standard succinyl-linker solid supports, without contamination of materials resulting from linker or support backbone decomposition. |
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Keywords: | Oligonucleotide Succinate Detritylation Universal solid support |
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