Synthesis of thiol-modified peptide nucleic acids designed for post-assembly conjugation reactions |
| |
Authors: | Martijn C. de Koning |
| |
Affiliation: | Leiden Institute of Chemistry, Leiden University, PO Box 9502, 2300 RA Leiden, The Netherlands |
| |
Abstract: | Two orthogonally protected PNA monomers were prepared having the mercaptomethyl moiety attached to the PNA backbone. These building blocks were employed in solid-phase PNA synthesis and it was shown that Boc/S-p-methoxybenzyl protection scheme was only satisfactory for the introduction of N-terminal thiol modification while the Fmoc/S-butylthio protected monomer proved to be amenable to elongation. The mercaptomethyl modification did not influence the thermal stability of a PNA/RNA duplex. The feasibility of PNA-PNA native ligation was demonstrated. |
| |
Keywords: | Peptide nucleic acid Conjugation Solid-phase synthesis |
本文献已被 ScienceDirect 等数据库收录! |