Enzyme electrodes using ultrafiltration membranes as enzyme carriers

Two kinds of commercially available ultrafiltration membranes were used for binding enzymes. These enzyme-bound membranes were connected with oxygen electrodes to build up enzyme electrodes. Quantitative assays of glucose, sucrose, and hydrogen peroxide were carried out using these enzyme electrodes in concentration ranges for glucose from 1 to 20 mg/dl, sucrose from 0.5 to 5 mg/ml, and hydrogen peroxide from 5 to 200 ppm. Similarly, D-alanine was measured in the range 0.005 to 0.05 mg/ml, but the calibration curve was not a straight line.