LB膜诱导下草酸钙晶体的生物矿化研究

草酸钙（CaC２O４）结石是生物体内异常矿化形成的产物，是泌尿系结石的主要组成部分犤１犦。它形成于泌尿系统中尿路表皮细胞上犤２犦。磷脂的Langmuir－Blodgett（LB）膜是生物细胞膜的简化模拟体系犤３犦。用磷脂LB膜作为模板来研究CaC２O４晶体的形成过程，比在水溶液中更能接近CaC２O４结石在生物体内细胞膜表面形成的真实情况，将有助于对尿路结石的形成及防治的研究犤４犦。尿大分子硫酸软骨素A（C４S）存在于尿液中，但不存在于CaC２O４结石中。在结石病人尿液中C４S的含量明显低于正常人尿液中的含量，因此推测其在体内…

The Study on the Biomineralization of Calcium Oxalate Crystals Induced by LB Film

The effects of urinary macromolecule chondroitin sulfate A(C₄S) and L-glutamic acid (L-Glu) on the crys-tallization of calcium oxalate were studied using Langmuir-Blodgett(LB) film of dipalmitoylphosphatidylcholine (DPPC) as templet. All the calcium oxalate crystals induced by the LB film of DPPC were calcium oxalate monohydrate(COM). However, the morphology of COM was influenced by the additives of C₄S and L-Glu. C₄S induced thin and long hexagonal COM crystals; L-Glu made one or two (010) crystal face of COM crystals dis-appeared. The crystallization time had no effect on the morphology of COM crystals, but the concentration of C₄S and L-Glu changed it. As increasing the concentration of C₄S, the amount of COM crystals with a hexagonal prism decreased and that with a thin hexagonal slice increased. When the concentration of C₄S was 0.50mg·mL^-1, all the calcium oxalate crystals were thin hexagonal slice COM crystals. However, as the concentration of L-Glu in-creased from 0.01 to 0.50mmol·L^-1, the hexagonal prism-like COM crystals gradually transformed to COM crystals with one or two (010) crystal face disappearance.