Purification of the isoflavonoid puerarin by adsorption chromatography on cross-linked 12% agarose |
| |
Authors: | He Xiangling Tan Tianwei Janson Jan-Christer |
| |
Affiliation: | Department of Biotechnology, School of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China. |
| |
Abstract: | The isoflavonoid puerarin in extracts of the well-known traditional Chinese drug Radix puerariae (root of the plant Pueraria lobata) can be separated from other isoflavonoids by adsorption chromatography on the cross-linked 12% agarose gel Superose 12 equilibrated in distilled water. The adsorption is totally quenched by the addition of 50% acetic acid. The separation of the isoflavonoids is tentatively ascribed to interaction with the residues of the cross-linking reagents used in the manufacturing process of Superose 12. Thus, no useful separation can be achieved with non-cross-linked 12% agarose gel media. Symmetric elution profiles at high sample loadings (16 mg on a 24 ml column) suggest linear adsorption isotherms for the isoflavonoids. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|